fabp7 polyclonal antibody  (Proteintech)

Journal: In Vivo

Article Title: FABP7: A Potential Diagnostic and Prognostic Biomarker for Clear Cell Renal Cell Carcinoma

doi: 10.21873/invivo.13773

Figure Lengend Snippet: The correlation of clinicopathologic features and FABP7 expression.

Article Snippet: The samples were stained with a FABP7 polyclonal antibody (dilution 1:100, Proteintech, Chicago, IL, USA).

Techniques: Expressing

Journal: In Vivo

Article Title: FABP7: A Potential Diagnostic and Prognostic Biomarker for Clear Cell Renal Cell Carcinoma

doi: 10.21873/invivo.13773

Figure Lengend Snippet: Expression of FABP7 in clear cell renal cell carcinoma (ccRCC) tissues compared with normal renal and non-ccRCC tissues (20´). A, C, E, G, and I) The illustration depicts histomorphology features with H&E staining of normal renal tissue, ccRCC, papillary RCC (pRCC), chromophobe RCC (chRCC), and rare subtypes RCC (rRCC). B, D, F, H, and J) The expression of FABP7 in normal renal tissue, ccRCC, pRCC, chRCC, and rRCC. B) The weak membranous and cytoplasmic staining of FABP7 in normal renal tubes. D) In ccRCC, FABP7 is expressed differently between low nuclear grade (low expression) and high nuclear grade (high expression). F, H, and J) FABP7 showed negative staining in non-ccRCC groups, including pRCC, chRCC, and rRCC.

Article Snippet: The samples were stained with a FABP7 polyclonal antibody (dilution 1:100, Proteintech, Chicago, IL, USA).

Techniques: Expressing, Staining, Negative Staining

Journal: In Vivo

Article Title: FABP7: A Potential Diagnostic and Prognostic Biomarker for Clear Cell Renal Cell Carcinoma

doi: 10.21873/invivo.13773

Figure Lengend Snippet: The gene distribution and the receiver operating characteristic curve of FABP7 expression between clear cell renal cell carcinoma (ccRCC) and non-ccRCC. A) The box plot diagram of FABP7 protein expression comparing ccRCC and non-ccRCC. The x-axis represents the groups of RCC, and the y-axis represents the gene expression values based on the immunohistology score (H-score). FABP7 increased in ccRCC groups compared with non-ccRCC. B) In the ROC curve and AUC analysis of FABP7 expression, the AUC value was moderate, 0.71. C) The box plots of FABP7 expression in three common subtypes of RCC compared with normal tissues. The x-axis represents the three common subtypes of RCC, including ccRCC, pRCC, and chRCC. The y-axis represents the FABBP7 expression based on log2 values. Expression of FABP7 mRNA in 523 ccRCC compared to 100 normal tissues was statistically significant. In pRCC, there is minimal FABP7 mRNA expression, although lower than that observed in ccRCC. There is no expression of FABP7 mRNA in chRCC. *p<0.05.

Article Snippet: The samples were stained with a FABP7 polyclonal antibody (dilution 1:100, Proteintech, Chicago, IL, USA).

Techniques: Expressing, Gene Expression

Journal: In Vivo

Article Title: FABP7: A Potential Diagnostic and Prognostic Biomarker for Clear Cell Renal Cell Carcinoma

doi: 10.21873/invivo.13773

Figure Lengend Snippet: The violin plots of the correlation between FABP7 and the pathological stage of renal cell carcinoma (RCC) in each subtype. A) ccRCC; the level of FABP7 expression was closely associated with the advanced pathological stages of clear cell RCC (ccRCC). B and C) pRCC and chRCC; the expression of FABP7 was not statistically significant. The x-axis represents the FABP7 expression; the y-axis represents the pathological stages. F Value: F-value is derived from an ANOVA test (Analysis of Variance) and is used to compare the means of the distributions to examined if they are statistically different from each other. An F-value greater than 1 suggests a variability between the group means. Pr(>F): This is the p-value derived from the ANOVA test. A p-value less than 0.05 indicates a statistically significant difference between the group means. The smaller the p-value, the stronger the evidence against the null hypothesis (which would be that there is no difference between the group means). White dot: This indicates the mean of the data.

Article Snippet: The samples were stained with a FABP7 polyclonal antibody (dilution 1:100, Proteintech, Chicago, IL, USA).

Techniques: Expressing, Derivative Assay

Journal: In Vivo

Article Title: FABP7: A Potential Diagnostic and Prognostic Biomarker for Clear Cell Renal Cell Carcinoma

doi: 10.21873/invivo.13773

Figure Lengend Snippet: The result of the Kaplan-Meier analysis of FABP7 expression in clear cell renal cell carcinoma (ccRCC) from the TCGA cohort: A) The overall survival analysis indicated no significant difference between the low and high expression of FABP7 in ccRCC patients; however, there was a trend toward significance [log-rank p=0.086; hazard ratio (HR)=1.3, p(HR)=0.087]. B) The disease-free survival analysis showed that high expression of FABP7 was associated with shortened survival in patients with ccRCC compared to those with low expression [log-rank p=0.008; HR=1.6; p(HR)=0.0087].

Article Snippet: The samples were stained with a FABP7 polyclonal antibody (dilution 1:100, Proteintech, Chicago, IL, USA).

Techniques: Expressing

Journal: Clinical Medicine Insights. Oncology

Article Title: Impact of Gender in Renal Cell Carcinoma: The Relationship of FABP7 and BRN2 Expression with Overall Survival

doi: 10.4137/CMO.S13684

Figure Lengend Snippet: Clinicopathological parameters (percentages in brackets) and mRNA and protein expression of FABP7 and BRN2 in renal cell carcinomas.

Article Snippet: For FABP7, slides were incubated overnight with a 1:100 dilution of the polyclonal antibody for FABP7 (catalog number HPA028825, Sigma-Aldrich, Inc, Stockholm, Sweden) in phosphate-buffered saline at 4 °C.

Techniques: Expressing

Journal: Clinical Medicine Insights. Oncology

Article Title: Impact of Gender in Renal Cell Carcinoma: The Relationship of FABP7 and BRN2 Expression with Overall Survival

doi: 10.4137/CMO.S13684

Figure Lengend Snippet: Kaplan-Meier survival curves for FABP7 (−) and BRN2 (+) mRNA and protein expression in women. FABP7 (−) and BRN2 (+) group (green line) revealed shorter survival times when compared with other patients (blue line) in mRNA level ( A ) and protein level ( B ). In mRNA data, there are 4 patients with FABP7 (−) and BRN2 (+), and the average survival time is 38.8 months, however, other 16 patients’ average survival time is 60.0 months. In protein data, there are 5 patients with FABP7 (−) and BRN2 (+), and the average survival time is 40.7 months, while, other 15 patients’ average survival time is 60.8 months.

Article Snippet: For FABP7, slides were incubated overnight with a 1:100 dilution of the polyclonal antibody for FABP7 (catalog number HPA028825, Sigma-Aldrich, Inc, Stockholm, Sweden) in phosphate-buffered saline at 4 °C.

Techniques: Expressing

Journal: Clinical Medicine Insights. Oncology

Article Title: Impact of Gender in Renal Cell Carcinoma: The Relationship of FABP7 and BRN2 Expression with Overall Survival

doi: 10.4137/CMO.S13684

Figure Lengend Snippet: FABP7 and BRN2 immunohistochemistry. ( A ) In normal tissue, FABP7 is preferentially expressed in the proximal tubuli. ( B ) In carcinomas tissue, FABP7 can be expressed in any part of the cell. ( C ) In normal tissue, BRN2 is expressed in all parts and can be strongly expressed in a few tubuli. ( D ) In carcinomas tissue, BRN2 is preferentially expressed in the nucleus.

Article Snippet: For FABP7, slides were incubated overnight with a 1:100 dilution of the polyclonal antibody for FABP7 (catalog number HPA028825, Sigma-Aldrich, Inc, Stockholm, Sweden) in phosphate-buffered saline at 4 °C.

Techniques: Immunohistochemistry

Journal: Clinical Medicine Insights. Oncology

Article Title: Impact of Gender in Renal Cell Carcinoma: The Relationship of FABP7 and BRN2 Expression with Overall Survival

doi: 10.4137/CMO.S13684

Figure Lengend Snippet: Kaplan-Meier survival curves for patients divided by protein level weighted score. ( A ) In men, 10 patients in the FABP7 (ws−) and BRN2 (ws+) group (green line) survived, and the other male patients are shown by the blue line. ( B ) In women, 8 patients in the FABP7 (ws+) and BRN2 (ws+) group (green line) survived, and the other female patients are shown by the blue line.

Article Snippet: For FABP7, slides were incubated overnight with a 1:100 dilution of the polyclonal antibody for FABP7 (catalog number HPA028825, Sigma-Aldrich, Inc, Stockholm, Sweden) in phosphate-buffered saline at 4 °C.

Techniques: